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Effect of CNDs-based mRNA transfection on expression levels of cell surface markers on Raw264.7 cells. Cells were treated with mLuc alone, CNDs alone, or mLuc–CNDs complexes. NT cells were used as a control. After 24 h post-treatment, cells were stained for <t>CD45</t> and CD11b and quantified using flow cytometry.
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Effect of CNDs-based mRNA transfection on expression levels of cell surface markers on Raw264.7 cells. Cells were treated with mLuc alone, CNDs alone, or mLuc–CNDs complexes. NT cells were used as a control. After 24 h post-treatment, cells were stained for CD45 and CD11b and quantified using flow cytometry.

Journal: ACS Omega

Article Title: Highly Efficient Messenger RNA Transfection of Hard-to-Transfect Cells using Carbon Nanodots

doi: 10.1021/acsomega.3c01394

Figure Lengend Snippet: Effect of CNDs-based mRNA transfection on expression levels of cell surface markers on Raw264.7 cells. Cells were treated with mLuc alone, CNDs alone, or mLuc–CNDs complexes. NT cells were used as a control. After 24 h post-treatment, cells were stained for CD45 and CD11b and quantified using flow cytometry.

Article Snippet: APC/Cy7 anti-mouse CD45 and PerCP/Cy5.5 anti-mouse CD11b were purchased from Biolegend.

Techniques: Transfection, Expressing, Control, Staining, Flow Cytometry